《植物生理学报》 2018, 54(10): 1587-1595

桃PpSnRK1α在番茄中超表达提高植株耐盐性

张淑辉, 罗静静, 陈晓璐, 王红, 肖元松^*, 彭福田^*

山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018

通信作者：肖元松；E-mail: ysxiao@sdau.edu.cn, pft@sdau.edu.cn

摘　要：

以超表达桃(Prunus persica)蔗糖非发酵蛋白激酶-1 (SnRK1)基因(PpSnRK1α)的番茄(Solanum lycopersicum)株系及野生型为试材, 研究盐胁迫下SnRK1对植株生长的影响。结果表明: 盐胁迫下, 番茄叶片和根系中SnRK1活性呈现先上升后下降的趋势, 但超表达PpSnRK1α番茄叶片和根系中SnRK1活性始终显著高于野生型植株; 正常条件下, 超表达PpSnRK1α番茄相比野生型根系活力提高了10.16%, 盐胁迫下提高了18.92%, 差异显著; 叶片伊文思蓝(Evans blue)染色结果发现, 超表达PpSnRK1α番茄叶片受伤害程度明显轻于野生型; 盐胁迫处理3、6、9和12 d后, 超表达PpSnRK1α番茄叶片过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均显著高于野生型植株, 而超氧阴离子(O₂ ^¯· )和丙二醛含量显著低于野生型植株; 盐胁迫处理4、8和12 d后, 超表达PpSnRK1α番茄叶片叶绿素含量、净光合速率(Pn)及最大光化学效率(Fv/Fm)的降低幅度显著低于野生型番茄。这些结果表明, 盐胁迫下, 超表达PpSnRK1α番茄植株比野生型植株SnRK1活性显著提高, 并通过提高植株的抗氧化能力和活性氧清除能力, 缓解盐胁迫对叶片叶绿素的降解和对光系统II (PSII)的破坏程度, 进而提高了植株的耐盐性。

关键词：PpSnRK1α; 番茄; 盐胁迫; 活性氧代谢; 根系活力; PSII

收稿：2018-07-25   修定：2018-09-15

资助：国家现代农业产业技术体系建设专项资金(CARS-30-2-02)、山东省自然科学基金(ZR2017BC017)和山东省“双一流”建设奖补资金(SYL2017YSTD10)。

Overexpression of a peach PpSnRK1α gene improving salt stress tolerance in tomato

ZHANG Shu-Hui, LUO Jing-Jing, CHEN Xiao-Lu, WANG Hong, XIAO Yuan-Song^*, PENG Fu-Tian^*

College of Horticulture Science and Engineering, Shandong Agricultural University; State Key Laboratory of Crop Biology, Taian, Shandong 271018, China

Corresponding author: XIAO Yuan-Song; E-mail: ysxiao@sdau.edu.cn, pft@sdau.edu.cn

Abstract:

PpSnRK1α-overexpressing tomato (Solanum lycopersicum) and wild type (WT) tomato were used to study the effect of sucrose non-fermenting-1-related protein kinase-1 (SnRK1) on the growth of tomato plants under salt stress. The results show that, under salt stress, the activities of SnRK1 in tomato leaves and roots showed a trend of first rising and then falling, but SnRK1 activities in the leaves and roots of PpSnRK1α-overexpressing lines were significantly higher than those of WT. Under normal conditions, PpSnRK1α-overexpressing lines increased the root activity by 10.16% compared to WT. Under salt stress, it’s 18.92% higher than WT, and the difference was significant. The results show that the damage degree of PpSnRK1α-overexpressing line leaves were significantly lower than those of WT by Evans blue dyeing. Under 3, 6, 9 and 12 days of salt stress, activities of catalase, superoxide dismutase and peroxidase in PpSnRK1α-overexpressing line leaves were significantly higher than those of WT, and O₂ ^¯· production and MDA content in PpSnRK1α-overexpressing line leaves significantly lower than those of WT. The reductions of chlorophyll content, net photosynthetic rate (Pn) and maximal photochemical efficiency of photosystem II (Fv/Fm) in PpSnRK1α-overexpressing lines were significantly less than those of WT. The results show that, under salt stress, SnRK1 activities in PpSnRK1α-overexpressing line leaves increased significantly than those of WT. In consequence, the abilities of oxidation resistance and reactive oxygen species removal in PpSnRK1α-overexpressing lines were improved, the degradation of chlorophyll and the degree of damage to the photosystem II were alleviated, and salt resistance of the plants was improved.

Key words: PpSnRK1α; tomato; salt stress; reactive oxygen species metabolism; root activity; PSII